Module Description

Module: Bioprocess Engineering - Advanced

Courses:

TitleTypeHrs/WeekPeriod
Bioprocess Engineering - AdvancedLecture2Winter Semester
Bioprocess Engineering - AdvancedRecitation Section (small)2Winter Semester

Module Responsibility:

Prof. An-Ping Zeng

Admission Requirements:

None

Recommended Previous Knowledge:

Content of module "Biochemical Engineering I"

Educational Objectives:

Professional Competence

Theoretical Knowledge

After successful completion of this module, students should be able to

  • describe and explain different kinetic approaches for growth and substrate-uptake
  •  identification of scientific problems with concrete industrial use (cultivation of microorganisms and mammalian cells)
  • describe and explain  important downstreaming steps for proteins and their application as well as basic immobilization methods
Capabilities

After successful completion of this module, students should be able to

- to identifiy scientific questions or possible practical problems for concrete industrial applications (eg cultivation of microorganisms and animal cells ) and to formulate solutions ,
- To assess the application of scale-up criteria for different types of bioreactors and processes and to apply these criteria to given problems (anaerobic , aerobic or microaerobically)
- to formulate questions for the analysis and optimization of real biotechnological production processes appropriate solutions ,
- To describe the effects of the energy generation, the regeneration of reduction equivalents , and the growth inhibition of the behavior of microorganisms and to the total fermentation process qualitatively


- Establish material flow balance equations and solve them to determine the kinetic parameters of different approaches and to calculate immobilization and activity yields ,
- to select process control strategies (batch , fed-batch , continuity ) appropriately and to  calculate basic types and evaluate them.

Personal Competence

Social Competence

After completion of this module participants should be able to debate technical questions in small teams to enhance the ability to take position to their own opinions and increase their capacity for teamwork. 

Autonomy

After completion of this module participants are able to aquire new sources of knowledge and apply their knowledge to previously unknown issues and to present these.

ECTS-Credit Points Module:

6 ECTS

Examination:

Written exam

Workload in Hours:

Independent Study Time: 124, Study Time in Lecture: 56


Course: Bioprocess Engineering - Advanced (Lecture)

Lecturers:

Andreas Liese, An-Ping Zeng

Language:

German

Period:

Winter Semester

Content:

  • Introduction: state-of-the-art and development trends of microbial and biocatalytic bioprocesses, introduction to the lecture  
  • Enzymatic process I: reactor types and criteria for industrial biotransformations (Prof. Liese)
  • Enzymatic process II (Prof. Liese)
  • Immobilization technologies: basic methods for isoltaed enzymes/ cells (Prof. Liese) 
  • Anaerobic fermentation processes (Prof. Zeng)
  • Microaerobic bioprocesses: kinetics, energetics, optimal O2-supply and scale-up (Prof. Zeng) 
  • Fedbatch process and cultivation with high cell density (Prof. Zeng)
  • Downstream processing of protein bioproduction: basics of chromatography, membrane filtration (Prof. Liese)
  • Cell culture technology and continuous culture: basics, kinetics, media, reactors (Prof. Zeng)
  • Problem-based learning with selected bioprocesses (Prof. Liese, Prof. Zeng) 

Literature:

K. Buchholz, V. Kasche, U. Bornscheuer: Biocatalysts and Enzyme Technology, 2. Aufl. Wiley-VCH, 2012

H. Chmiel: Bioprozeßtechnik, Elsevier, 2006

R.H. Balz et al.: Manual of Industrial Microbiology and Biotechnology, 3. edition, ASM Press, 2010 

H.W. Blanch, D. Clark: Biochemical Engineering, Taylor & Francis, 1997 

P. M. Doran: Bioprocess Engineering Principles, 2. edition, Academic Press, 2013


Skripte für die Vorlesung


Course: Bioprocess Engineering - Advanced (Recitation Section (small))

Lecturer:

An-Ping Zeng

Language:

German

Period:

Winter Semester

Content:

  • Introduction: state-of-the-art and development trends of microbial and biocatalytic bioprocesses, introduction to the lecture  
  • Enzymatic process I: reactor types and criteria for industrial biotransformations (Prof. Liese)
  • Enzymatic process II (Prof. Liese)
  • Immobilization technologies: basic methods for isoltaed enzymes/ cells (Prof. Liese) 
  • Anaerobic fermentation processes (Prof. Zeng)
  • Microaerobic bioprocesses: kinetics, energetics, optimal O2-supply and scale-up (Prof. Zeng) 
  • Fedbatch process and cultivation with high cell density (Prof. Zeng)
  • Downstream processing of protein bioproduction: basics of chromatography, membrane filtration (Prof. Liese)
  • Cell culture technology and continuous culture: basics, kinetics, media, reactors (Prof. Zeng)
  • Problem-based learning with selected bioprocesses (Prof. Liese, Prof. Zeng) 

The students present exercises and discuss them with their fellow students and faculty statt. In the PBL part of the class the students discuss scientific questions in teams. They acquire knowledge and apply it to unknown questions, present their results and argue their opinions.

Literature:

K. Buchholz, V. Kasche, U. Bornscheuer: Biocatalysts and Enzyme Technology, 2. Aufl. Wiley-VCH, 2012

H. Chmiel: Bioprozeßtechnik, Elsevier, 2006

R.H. Balz et al.: Manual of Industrial Microbiology and Biotechnology, 3. edition, ASM Press, 2010 

H.W. Blanch, D. Clark: Biochemical Engineering, Taylor & Francis, 1997 

P. M. Doran: Bioprocess Engineering Principles, 2. edition, Academic Press, 2013


Skripte für die Vorlesung