Module Description

Module: Biocatalysis

Courses:

TitleTypeHrs/WeekPeriod
Biocatalysis and Enzyme TechnologyLecture2Winter Semester
Technical BiocatalysisLecture2Winter Semester

Module Responsibility:

Prof. Andreas Liese

Admission Requirements:

None

Recommended Previous Knowledge:

Knowledge of bioprocess engineering and process engineering at bachelor level

Educational Objectives:

Professional Competence

Theoretical Knowledge

After successful completion of this course, students will be able to

  • reflect a broad knowledge about enzymes and their applications in academia and industry
  • have an overview of relevant biotransformations und name the general definitions
Capabilities

After successful completion of this course, students will be able to

  • understand the fundamentals of biocatalysis and enzyme processes and transfer this to new tasks
  • know the several enzyme reactors and the important parameters of enzyme processes
  • use their gained knowledge about the realisation of processes. Transfer this to new tasks
  • analyse and discuss special tasks of processes in plenum and give solutions
  • communicate and discuss in English

Personal Competence

Social Competence

After completion of this module, participants will be able to debate technical and biocatalytical questions in small teams to enhance the ability to take position to their own opinions and increase their capacity for teamwork. 

Autonomy

After completion of this module, participants will be able to solve a technical problem independently including a presentation of the results.

ECTS-Credit Points Module:

6 ECTS

Examination:

Written exam

Workload in Hours:

Independent Study Time: 124, Study Time in Lecture: 56


Course: Biocatalysis and Enzyme Technology (Lecture)

Lecturer:

Andreas Liese

Language:

English

Period:

Winter Semester

Content:

1. Introduction: Impact and potential of enzyme-catalysed processes in biotechnology.

2. History of microbial and enzymatic biotransformations.

3. Chirality - definition & measurement

4. Basic biochemical reactions, structure and function of enzymes.

5. Biocatalytic retrosynthesis of asymmetric molecules

6. Enzyme kinetics: mechanisms, calculations, multisubstrate reactions.

7. Reactors for biotransformations.

Literature:

  • K. Faber: Biotransformations in Organic Chemistry, Springer, 5th Ed., 2004
  • A. Liese, K. Seelbach, C. Wandrey: Industrial Biotransformations, Wiley-VCH, 2006
  • R. B. Silverman: The Organic Chemistry of Enzyme-Catalysed Reactions, Academic Press, 2000
  • K. Buchholz, V. Kasche, U. Bornscheuer: Biocatalysts and Enzyme Technology. VCH, 2005.
  • R. D. Schmidt: Pocket Guide to Biotechnology and Genetic Engineering, Woley-VCH, 2003

Course: Technical Biocatalysis (Lecture)

Lecturer:

Andreas Liese

Language:

English

Period:

Winter Semester

Content:

1. Introduction

2. Production and Down Stream Processing of Biocatalysts

3. Analytics (offline/online)

4. Reaction Engineering & Process Control

  • Definitions
  • Reactors
  • Membrane Processes
  • Immobilization

5. Process Optimization

  • Simplex / DOE / GA

6. Examples of Industrial Processes

  • food / feed
  • fine chemicals

7. Non-Aqueous Solvents as Reaction Media

  • ionic liquids
  • scCO2
  • solvent free

Literature:

  •  A. Liese, K. Seelbach, C. Wandrey: Industrial Biotransformations, Wiley-VCH, 2006
  •  H. Chmiel: Bioprozeßtechnik, Elsevier, 2005
  •  K. Buchholz, V. Kasche, U. Bornscheuer: Biocatalysts and Enzyme Technology, VCH, 2005
  •  R. D. Schmidt: Pocket Guide to Biotechnology and Genetic Engineering, Woley-VCH, 2003