Module Description

Module: Separation Technologies for Life Sciences

Courses:

TitleTypeHrs/WeekPeriod
Chromatographic Separation ProcessesLecture2Winter Semester
Unit Operations for Bio-Related SystemsLecture2Winter Semester
Unit Operations for Bio-Related SystemsProject-/problem-based Learning2Winter Semester

Module Responsibility:

Prof. Irina Smirnova

Admission Requirements:

None

Recommended Previous Knowledge:

Fundamentals of Chemistry, Fluid Process Engineering, Thermal Separation Processes, Chemical Engineering, Chemical Engineering, Bioprocess Engineering

Basic knowledge in thermodynamics and in unit operations related to thermal separation processes

Educational Objectives:

Professional Competence

Theoretical Knowledge

On completion of the module, students are able to present an overview of the basic thermal process technology operations that are used, in particular, in the separation and purification of biochemically manufactured products. Students can describe chromatographic separation techniques and classic and new basic operations in thermal process technology and their areas of use. In their choice of separation operation students are able to take the specific properties and limitations of biomolecules into consideration. Using different phase diagrams they can explain the principle behind the basic operation and its suitability for bioseparation problems.

Capabilities

On completion of the module, students are able to assess the separation processes for bio- and pharmaceutical products that have been dealt with for their suitability for a specific separation problem. They can use simulation software to establish the productivity and economic efficiency of bioseparation processes. In small groups they are able to jointly design a downstream process and to present their findings in plenary and summarize them in a joint report.

Personal Competence

Social Competence

Students are able in small heterogeneous groups to jointly devise a solution to a technical problem by using project management methods such as keeping minutes and sharing tasks and information.

Autonomy

Students are able to prepare for a group assignment by working their way into a given problem on their own. They can procure the necessary information from suitable literature sources and assess its quality themselves. They are also capable of independently preparing the information gained in a way that all participants can understand (by means of reports, minutes, and presentations).

ECTS-Credit Points Module:

6 ECTS

Examination:

Written exam

Workload in Hours:

Independent Study Time: 96, Study Time in Lecture: 84


Course: Chromatographic Separation Processes (Lecture)

Lecturer:

Monika Johannsen

Language:

English

Period:

Winter Semester

Content:

  • Introduction: overview, history of chromatography, LC (HPLC), GC, SFC
  • Fundamentals of linear (analytical) chromatography, retention time/factor, separation factor, peak resolution, band broadening, Van-Deemter equation
  • Fundamentals of nonlinear chromatography, discontinuous and continuous preparative chromatography (annular, true moving bed - TMB, simulated moving bed - SMB)
  • Adsorption equilibrium: experimental determination of adsorption isotherms and modeling
  • Equipment for chromatography, production and characterization of chromatographic adsorbents
  • Method development, scale up methods, process design, modeling of chromatographic processes, economic aspects
  • Applications: e.g. normal phase chromatography, reversed phase chromatography, hydrophobic interaction chromatography, chiral chromatography, bioaffinity chromatography, ion exchange chromatography

Literature:

  • Schmidt-Traub, H.: Preparative Chromatography of Fine Chemicals and Pharmaceutical Agents. Weinheim: Wiley-VCH (2005) - eBook
  • Carta, G.: Protein chromatography: process development and scale-up. Weinheim: Wiley-VCH (2010)
  • Guiochon, G.; Lin, B.: Modeling for Preparative Chromatography. Amsterdam: Elsevier (2003)
  • Hagel, L.: Handbook of process chromatography: development, manufacturing, validation and economics. London ;Burlington, MA Academic (2008) - eBook

Course: Unit Operations for Bio-Related Systems

Lecturer:

Irina Smirnova

Language:

English

Period:

Winter Semester

Content:

Contents:

  • Introduction: overview about the separation process in biotechnology and pharmacy
  • Handling of multicomponent systems
  • Adsorption of biologic molecules
  • Crystallization of biologic molecules
  • Reactive extraction
  • Aqueous two-phase systems
  • Micellar systems: micellar extraction and micellar chromatographie
  • Electrophoresis
  •  Choice of the separation process for the specific systems

Learning Outcomes:

  • Basic knowledge of separation processes for biotechnological and pharmaceutical processes
  • Identification of specific features and limitations in bio-related systems
  • Proof of economical value of the process

Literature:

"Handbook of Bioseparations", Ed. S. Ahuja

http://www.elsevier.com/b … 2/ahuja/978-0-12-045540-9

"Bioseparations Engineering" M. R. Ladish

http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0471244767.html